Characterizing Complex Cell Populations: The Power of 10x Chromium Lysis Buffer

The ability to characterize complex cell populations has revolutionized the field of biology and medicine. With the advent of single-cell RNA sequencing (scRNA-seq) technologies, researchers can now gain insights into the heterogeneity of cells within a population, providing a deeper understanding of biological processes such as development, immunity, and pathology.

At the forefront of scRNA-seq technology is the 10x Chromium Lysis Buffer, which has become a cornerstone for characterizing complex cell populations. This buffer plays a crucial role in preparing samples for downstream processing and analysis.

Sample Preparation

When working with complex cell populations, it's essential to carefully prepare the sample to ensure optimal results. The recommended concentration of cells in PBS with 0.04% BSA is 1,000 cells/μl. It's also important to monitor cell viability and remove any clumps or debris before proceeding.

10x Chromium Lysis Buffer: A Key Component

The 10x Chromium Lysis Buffer is a critical component in the scRNA-seq process. This buffer is designed to lyse cells and release their RNA, which can then be sequenced using the Drop-Seq platform from 10X Genomics. The buffer is sensitive to cell viability and requires careful handling to ensure optimal results.

Features of the 10x Chromium Lysis Buffer

The 10x Chromium Lysis Buffer offers several key features that make it an ideal choice for characterizing complex cell populations:

* Sensitive detection: The buffer allows for the detection of 1,000-4,000 genes per cell, providing a high level of resolution in gene expression profiling.

* High-throughput analysis: The Drop-Seq platform can analyze up to 10,000 cells per sample, identifying around 1,000-5,000 different transcripts per cell.

* Nano-reactor technology: Each droplet is a nano-reactor that contains only one cell and the reagents necessary for RNA extraction and amplification.

Applications of the 10x Chromium Lysis Buffer

The 10x Chromium Lysis Buffer has numerous applications in biology and medicine, including:

* Characterization of complex cell populations: The buffer is ideal for characterizing complex cell populations such as tumour samples, discovering new cell types, and identifying new markers to define cell populations.

* Gene expression profiling: The buffer allows for the detection of 1,000-4,000 genes per cell, providing a high level of resolution in gene expression profiling.

Access Mode

The 10x Chromium Lysis Buffer is available with walk-in access and full service for users with up to three runs. Our team will process your samples and provide you with the results you need.

In conclusion, the 10x Chromium Lysis Buffer is a powerful tool for characterizing complex cell populations. Its sensitive detection capabilities, high-throughput analysis, and nano-reactor technology make it an ideal choice for researchers seeking to gain insights into biological processes. With its numerous applications in biology and medicine, the 10x Chromium Lysis Buffer has revolutionized the field of single-cell RNA sequencing.

10x chromium lysis buffer